Hirofumi TodaHirofumi Toda

Affiliation:International Institute for Integrative Sleep Medicine, University of Tsukuba

Research Title

Neuroscience

How is the sleepiness represented inside a brain?
~The power of using fruit flies in sleep research

01 Research Summary

The main goal of our lab is to understand the molecular and cellular mechanisms of sleep. Sleep is important and conserved in all animals from humans to insects. Yet, the function of sleep: why we need it, and the mechanisms of sleep: how it occurs remains largely mystery. In order to understand the mechanisms of sleep, we use fruit flies as a model system to tackle such questions because flies are fantastic genetic model organisms especially amenable for genetic screening. For example, to identify molecular factors that induces sleep, we carried out a genome-wide behavioral screen, evaluating over 12,000 fly lines, and found a novel sleep inducing factor named “nemuri”. Nemuri functions as an anti-microbial peptide that is essential for bacterial infection-induced sleep, suggesting Nemuri served as a key link between sleep and immunity. Our group combines genetic, biochemical, behavioral and imaging techniques to elucidate the mechanisms of how Nemuri functions inside neurons to induce sleep. Moreover, we aim to identify the mammalian homologue of nemuri through behavioral screening by expressing human candidate genes in fly brain. These studies will improve our understanding of how the sleepiness is represented inside the brain.

02 Major achievements

A sleep-inducing gene, nemuri, links sleep and immune function in Drosophila. Toda H, Williams JA, Gulledge M, Sehgal A. Science. 2019 Feb 1;363(6426):509-515. doi: 10.1126/science.aat1650.

The Drosophila female aphrodisiac pheromone activates ppk23(+) sensory neurons to elicit male courtship behavior. Toda H, Zhao X, Dickson BJ. Cell Rep. 2012 Jun 28;1(6):599-607. doi: 10.1016/j.celrep.2012.05.007. Epub 2012 May 24.

UNC-51/ATG1 kinase regulates axonal transport by mediating motor-cargo assembly. Toda H, Mochizuki H, Flores R 3rd, Josowitz R, Krasieva TB, Lamorte VJ, Suzuki E, Gindhart JG, Furukubo-Tokunaga K, Tomoda T. Genes Dev. 2008 Dec 1;22(23):3292-307. doi: 10.1101/gad.1734608.

03 Education/Academic background

2004-2009 D.Sc. Department of Biological Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba
2010-2013 Postdoctoral fellow at Institute of Molecular Pathology, Vienna, AT
2013-2016 Research Associate at HHMI/University of Pennsylvania, PA, USA
2016-2019 Research Specialist at HHMI/ University of Pennsylvania, PA, USA
2019-present Assistant Professor, International Institute for Integrative Sleep Medicine, University of Tsukuba